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Effects of ethanolic extract of Artemisia sieberi Besser on DNA glycation of glucose: Possible antidiabetic mechanism
Parisa Hasanein1 , Farnush Sotudeh , Mousa Bohlooli , Mohammad Haddadi
1- , parisa.hasanein@gmail.com
Abstract:   (504 Views)

Introduction: DNA Glycation damages DNA by inducing breaks of strands, mutations, and finally changes in gene expression, which is assumed as a main factor in pathogenesis of diabetes and its complications. Therefore, antiglycation agents have been focused recently for preventing and alleviating diabetes complications. According to the reported antidiabetic effects of Artemisia sieberi (A. sieberi) leaves extract, this study was aimed to determine the effect of ethanoic extract of A. sieberi on glucose-mediated DNA glycation for the first time.

Methods: DNA incubated with glucose in the presence or absence of A. sieberi for 4 weeks. The inhibitory or fascilitatory effects of A. sieberi on DNA structural changes were studied by various techniques. These techniques were included UV–Vis, fluorescence spectroscopy, circular dichroism (CD) and agarose gel electrophoresis.
Results: The findings of UV–Vis and fluorescence spectroscopy showed that A. sieberi decreased the DNA-AGE (advanced glycation end products) formation. Based on the CD and agarose gel electrophoresis results, the structural changes of glycated DNA was decreased in the presence of A. sieberi.
Keywords: Glycation, Artemisia sieberi, AGE, DNA, Glucose
Full-Text [PDF 445 kb]   (185 Downloads)    
Research Article: Research Article | Subject: Biochemistry
Received: 2025/03/8 | Accepted: 2025/08/27
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