Wed, Sep 23, 2026
1- Department of Biology, Faculty of science, Bu-Ali Sina University, Hamedan, Iran
2- Department of Biology, Faculty of science, Bu-Ali Sina University, Hamedan, Iran ,s.yari@basu.ac.ir
2- Department of Biology, Faculty of science, Bu-Ali Sina University, Hamedan, Iran ,
Abstract: (1366 Views)
Background: Chemical analgesics often have limitations, including adverse effects and insufficient efficacy. Therefore, there is a pressing need to investigate novel compounds with enhanced efficacy and a more favorable side effect profile.
Methods: This study aimed to evaluate the analgesic properties of Rhaponticum repens (RR) extract, a natural compound, using the formalin test in Wistar rats. Twenty-one male Wistar rats were randomly divided into three groups (n=7): group 1 received normal saline, group 2 received morphine (10 mg/kg), and group 3 received RR extract (100 mg/kg). Thirty minutes post-injection, formalin (2.5%) was injected into the hind paw of each rat to induce pain. Licking time and pain scores were recorded during both the acute and chronic phases of the test. Data were analyzed using one-way ANOVA with Prism software, with statistical significance set at P ≤ 0.05.
Results: Results from both the acute and chronic phases demonstrated a significant reduction in licking time and pain scores in both the morphine and RR extract groups compared to the control group. Additionally, the extent of hind paw edema was significantly reduced in both the morphine and RR extract groups.
Methods: This study aimed to evaluate the analgesic properties of Rhaponticum repens (RR) extract, a natural compound, using the formalin test in Wistar rats. Twenty-one male Wistar rats were randomly divided into three groups (n=7): group 1 received normal saline, group 2 received morphine (10 mg/kg), and group 3 received RR extract (100 mg/kg). Thirty minutes post-injection, formalin (2.5%) was injected into the hind paw of each rat to induce pain. Licking time and pain scores were recorded during both the acute and chronic phases of the test. Data were analyzed using one-way ANOVA with Prism software, with statistical significance set at P ≤ 0.05.
Results: Results from both the acute and chronic phases demonstrated a significant reduction in licking time and pain scores in both the morphine and RR extract groups compared to the control group. Additionally, the extent of hind paw edema was significantly reduced in both the morphine and RR extract groups.
References
1. Benzon H, Raja SN, Fishman SM, Liu SS, Cohen SP. Essentials of pain medicine. 3rd ed. Philadelphia: Elsevier Health Sciences; 2011. [View at Publisher] [DOI] [Google Scholar]
2. Fillingim RB, Loeser JD, Baron R, Edwards RR. Assessment of chronic pain: domains, methods, and mechanisms. The journal of pain. 2016;17(9):T10-T20. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Fitzcharles M-A, Cohen SP, Clauw DJ, Littlejohn G, Usui C, Häuser W. Nociplastic pain: towards an understanding of prevalent pain conditions. Lancet. 2021;397(10289):2098-110. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Diers M, Krumm B, Fuchs X, Bekrater-Bodmann R, Milde C, Trojan J, et al. The prevalence and characteristics of phantom limb pain and Non-Painful phantom phenomena in a nationwide survey of 3,374 unilateral limb amputees. J Pain. 2022;23(3):411-23. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Abubakar AR, Haque M. Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. J Pharm Bioallied Sci. 2020;12(1):1-10. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Farahani F, Azizi H, Janahmadi M, Seutin V, Semnanian S. Formalin-induced inflammatory pain increases excitability in locus coeruleus neurons. Brain Res Bull. 2021;172:52-60. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Akhgari Z, Tanomand A, Nazari R, Zargar M. Biological and Antibacterial Features of Acroptilon repens (L.) DC. Crescent j. med. biol. sci. 2022;9(2):116–22. [View at Publisher] [DOI] [Google Scholar]
8. Bai X, Tang J, Safaralizadeh R, Feizi MA, Delirezh N, Zangeneh A, et al. Green fabrication of bioactive copper nanoparticles using Acroptilon repens extract: An enhanced anti-lung cancer activity. Inorg Chem Commun. 2024;164:112393. [View at Publisher] [DOI] [Google Scholar]
9. Xu H, Blair NT, Clapham DE. Camphor activates and strongly desensitizes the transient receptor potential vanilloid subtype 1 channel in a vanilloid-independent mechanism. J Neurosci. 2005;25(39):8924-37. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Nwozo OS, Effiong EM, Aja PM, Awuchi CG. Antioxidant, phytochemical, and therapeutic properties of medicinal plants: A review. Int J Food Prop. 2023;26(1):359-88. [View at Publisher] [DOI] [Google Scholar]
11. Olennikov DN. The ethnopharmacological uses, metabolite diversity, and bioactivity of Rhaponticum uniflorum (Leuzea uniflora): A comprehensive review. Biomolecules. 2022;12(11):1720. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Sawynok J, Liu XJ. The formalin test: characteristics and usefulness of the model. Rev Analg. 2003;7(2):145-63. [View at Publisher] [DOI] [Google Scholar]
13. Paige C, Barba-Escobedo PA, Mecklenburg J, Patil M, Goffin V, Grattan DR, et al. Neuroendocrine Mechanisms Governing Sex Differences in Hyperalgesic Priming Involve Prolactin Receptor Sensory Neuron Signaling. J Neurosci. 2020;40(37):7080-90. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Clavelou P, Dallel R, Orliaguet T, Woda A, Raboisson P. The orofacial formalin test in rats: effects of different formalin concentrations. Pain. 1995;62(3):295-301. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Hoodgar F, Nasri S, Amin G. Investigation of antinociceptive and anti-inflammatory effects of hydro-alcoholic extract of Securigera Securidaca L. Intern Med Today. 2011;17(1):12-9. [View at Publisher] [Google Scholar]
16. Abbott FV, Franklin KB, Westbrook RF. The formalin test: scoring properties of the first and second phases of the pain response in rats. Pain. 1995;60(1):91-102. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Bannon AW, Malmberg AB. Models of nociception: hot-plate, tail-flick, and formalin tests in rodents. Curr Protoc Neurosci. 2007;Chapter 8:Unit 8.9. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Ramírez-Maestre C, Reyes-Pérez Á, Esteve R, López-Martínez AE, Bernardes S, Jensen MP. Opioid pain medication prescription for chronic pain in primary care centers: The roles of pain acceptance, pain intensity, depressive symptoms, pain catastrophizing, sex, and age. Int J Environ Res Public Health. 2020;17(17):6428. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Khumalo GP, Van Wyk BE, Feng Y, Cock IE. A review of the traditional use of southern African medicinal plants for the treatment of inflammation and inflammatory pain. J Ethnopharmacol. 2022;283:114436. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Abdolrazaghnejad A, Banaie M, Tavakoli N, Safdari M, Rajabpour-Sanati A. Pain management in the emergency department: a review article on options and methods. Adv J Emerg Med. 2018;2(4):e45. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Stevens KL, Riopelle RJ, Wong RY. Repin, a sesquiterpene lactone from Acroptilon repens possessing exceptional biological activity. J Nat Prod. 1990;53(1):218-21. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Afsharnezhad M, Shahangian SS, Rasti B, Ghasemi MF. Inhibitory potential of Acroptilon repens against key enzymes involved in alzheimer and diabetes, phytochemical profile, radical scavenging, and antibacterial activity. Iran Biomed J. 2021;25(1):21-32. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Dashti A, Shokrzadeh M, Karami M, Habibi E. Phytochemical identification, acute and subchronic oral toxicity assessments of hydroalcoholic extract of Acroptilon repens in BALB/c mice: A toxicological and mechanistic study. Heliyon. 2022;8(2):e08940. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Hwang D, Fischer NH, Jang BC, Tak H, Kim JK, Lee W. Inhibition of the expression of inducible cyclooxygenase and proinflammatory cytokines by sesquiterpene lactones in macrophages correlates with the inhibition of MAP kinases. Biochem Biophys Res Commun. 1996;226(3):810-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Hafez Ghoran S, Taktaz F, Mozafari AA, Tunçtürk M, Sekeroglu N, Kijjoa A. Uncommon Terpenoids from Salvia Species: Chemistry, Biosynthesis and Biological Activities. Molecules. 2022;27(3):1128. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Eidi A. Antinociceptive effects of ethanolic extract of salvia aethiopis in NMRI mice. Armaghane Danesh. 2014;19(1):24-35. [View at Publisher] [Google Scholar]
27. Mahmoudi M, Mohammadi S, Shahidi S. Antinociceptive effect of hydroalcoholic leaf extract of Hedera helix in male rat. Avicenna J Clin Med. 2013;20(2):119-25. [View at Publisher] [Google Scholar]
28. Alimohammadi B, Azhdari Zarmehri H, Dolatshahi M. The antinociceptive effect of hydroalcoholic extracts of Scrophularia striata Boiss. On mice using hot plate test. jmsthums. 2015;3(3):7-1. [View at Publisher] [Google Scholar]
29. Dashti Rahmatabadi M.H, Vahidi Mehrjardi A.R, Pilehvarian A.A, farzan F. Antinociceptive effect of cinnamon extract on formalin induced pain in rat. J Shahid Sadoughi Univ Med Sci. 2009;2(17):57-66. [View at Publisher] [Google Scholar]
30. Dos Santos E, Leitão MM, Ito CNA, Silva-Filho SE, Arena AC, de Souza Silva-Comar FM, et al. Analgesic and anti-inflammatory articular effects of essential oil and camphor isolated from Ocimum kilimandscharicum Gürke leaves. J Ethnopharmacol. 2021;269:113697. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Bouhlali EDT, Derouich M, Hmidani A, Bourkhis B, Khouya T, Filali-Zegzouti Y, et al. Protective Effect of Phoenix dactylifera L. Seeds against Paracetamol‐Induced Hepatotoxicity in Rats: A Comparison with Vitamin C. ScientificWorldJournal. 2021;2021(1):6618273. [View at Publisher] [DOI] [PMID] [Google Scholar]
32. Jayaraman S, Roy A, Vengadassalapathy S, Sekar R, Veeraraghavan VP, Rajagopal P, et al. An Overview on the Therapeutic Function of Foods Enriched with Plant Sterols in Diabetes Management. Antioxidants (Basel). 2021;10(12):1903. [View at Publisher] [DOI] [PMID] [Google Scholar]
33. Kabir MT, Rahman MH, Shah M, Jamiruddin MR, Basak D, Al-Harrasi A, et al. Therapeutic promise of carotenoids as antioxidants and anti-inflammatory agents in neurodegenerative disorders. Biomed Pharmacother. 2022;146:112610. [View at Publisher] [DOI] [PMID] [Google Scholar]
34. Taherian AA, Rashidipour A, Vafaei A, JArahi M, Miladi GH, Emami AM, et al. Effect of aqueous extract of the Coriandrum sativum seed on the reduction of acute and chronic pain in mice. J Rafsanjan Univ Med Sci. 2004;3(4);243-9. [View at Publisher] [Google Scholar]
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