Medical Laboratory Journal
Medical Laboratory Journal
mljgoums
Medical Sciences
http://mlj.goums.ac.ir
1
admin
2538-4449
2538-4449
10.66224/mlj
en
jalali
1396
4
1
gregorian
2017
7
1
11
4
online
1
fulltext
fa
Cytotoxic Effect of Capparis spinosa L. on the PLC/PRF/5 Human Hepatocellular Carcinoma Cell Line
تحقيقي
Original Paper
<div><strong>ABSTRACT</strong><br>
<strong> Background and Objectives: </strong><em>Capparis spinosa</em> has been used in traditional medicine for various applications including treatment of liver disorders and cancer. We studied the effects of this plant on cell proliferation and morphological characteristics of <em>PLC/PRF/5</em> liver cancer cell line.<br>
<strong> Methods: </strong>After preparing ethanolic extract of the plant, the inhibitory effect of the extract was assessed using MTT assay, and morphological changes were assessed by an inverted microscope.<br>
<strong> Results: </strong><em>C. spinosa</em> ethanolic extract exhibited anti-cancer effects in a concentration-dependent manner. Half-maximal inhibitory concentration of the extract was 1051±4.21 mg/mL. Morphological changes including cell shrinkage, reduction of cell volume and nuclear condensation confirmed the inhibitory effect of <em>C. spinosa</em> on <em>PLC/PRF/5</em> cells.<br>
<strong> Conclusion: </strong>According to the results of this study, extract of C. spinosa seems to be suitable for prevention and treatment of liver cancer. Further studies on animal models could verify the efficiency of the extract against cancer cells.
<h1><span style="font-size:10px;">KEYWORDS: Plants, Medicinal, <em>Capparis spinosa</em>, <a href="https://www.ncbi.nlm.nih.gov/mesh/68008113">Liver Neoplasms</a>.</span> <span dir="RTL"></span></h1>
</div>
Plants, Medicinal, Capparis spinosa, Liver Neoplasms.
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http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-467&slc_lang=fa&sid=1
Masoud
Sheikh