en انگل شناسی Parasitology مروري Review Article <span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background and objectives</span></span></span></b><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">: Trichomoniasis is considered <span style="color:red">as</span> the most common non-viral sexually transmitted <span style="color:red">diseases</span> <span style="color:red">globally</span>. Early diagnosis of <i>Trichomonas vaginalis</i> infection <span style="color:red">has </span>an <span style="color:red">important role in</span></span></span></span> <span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:red">effective treatment and controlling</span></span></span></span><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> the infection and interrupt of transmission chain. The present <span style="color:red">narrative review</span> was carried out to gather new data and evaluate the challenges of available methods of laboratory of <i><span style="color:red">T. vaginalis</span></i> <span style="color:red">diagnosis.</span></span></span></span><span lang="FA" style="font-size:13.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Methods: </span></span></span></b><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">With a broad review of the published literature and electronic <span style="color:red">international</span> databases such as <span style="color:red">ISI Web of Science,</span> PubMed, Scopus, Science Direct, and Google Scholar</span></span></span> <span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">was conducted</span></span></span> <span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""><span style="color:red">to find relevant data reporting on existing and newly developed diagnostic methods for the identification of trichomoniasis diagnostic techniques in both sexes</span></span></span></span><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> <span style="color:red">and covered 2000 up to 2025. The benefit and limitation of these methods were compared. </span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results: </span></span></span></b><span dir="LTR" style="font-size:13.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">This review article demonstrated <span style="color:red">wet mount preparation and examination under a light microscope accompanied by staining methods are the most frequently used worldwide. However, the sensitivity of this method is low</span>. <span style="color:red">Direct microscopy examination accompanied by culturing or staining is a good-performance diagnostic strategy. Moreover, molecular and immunological-based methods are recommended to be used as complementary tests. </span></span></span></span></span></span></span></span></span><br> <b><span style="font-size:13.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:13.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> Direct microscopy examination is an economical and rapid method. The fully automated PCR system for the simultaneous identification of the most common vaginal pathogens can be established as a diagnostic strategy in non-endemic areas and developed countries. </span></span></span> Trichomonas infection, Trichomonas vaginalis, Diagnosis, Laboratories 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-2157-1&slc_lang=en&sid=1 Hossein Hooshyar hooshyar4@yahoo.com 100319475328460032430 100319475328460032430 Yes Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran Parvin Rostamkhani rostamkh2002@yahoo.com 100319475328460032431 100319475328460032431 No Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran Mohsen Arbabi arbabi4.mohsen@yahoo.com 100319475328460032432 100319475328460032432 No Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran