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Comparison of Six Culture Methods for Salmonella Isolation from Poultry Fecal Samples
R Morshed,
Volume 8, Issue 1 (4-2014)
Abstract
Abstract Background and Objective: Salmonellosis is one of the most important food-borne bacterial zoonotic diseases worldwide, and poultry and its products are the major sources for salmonella transmission to human. Isolation of Salmonellaenterica from poultry needs bacteriologic enrichment and selected cultures of fecal samples. In this study, different culture methods for the isolation of salmonella from fecal samples were compared. Material and Methods: Forty- five positive samples from infected farms and 45 negative samples from normal farms were processed using enrichment media including tetrathionate broth, selenite cistine and Rappaport-Vassiliadis. Then the samples were incubated in selective cultures, and after 24 h, their results were compared with standard method. Results: Specificity of all methods for salmonella isolation was 100%, and salmonella was not isolated from the negative samples. The highest susceptibility was related to the method in which the sample first in Selenite cistine and later in Rappaport-Vassiliadis was enriched (100%). Enrichment in Rappaport-Vassiliadis could isolate 41 salmonella from 45 positive samples (91%) while the result of enrichment in tetrathionate was 6 isolates (13.3%). Conclusion: This study shows that enrichment in selenite cistine and then in Rappaport-Vassiliadis is currently the best method for isolating salmonella from fecal samples of poultry. Key words: Salmonella Bacteriologic Culture Diagnosis Isolation Enrichment Poultry
Characterization and optimization of keratinase production by bacillus pumilus and bacillus tequilensis isolated from poultry waste
Bahareh Behfar , Fatemeh Haddadi , Mohammad Reza Sharifmoghadam , Hossein Kamaladini, Masoumeh Bahreini, Azadeh Niknejad ,
Volume 20, Issue 2 (3-2026)
Abstract
Background: The poultry industry produces a large amount of waste, including chicken feathers, which are difficult to decompose and can cause environmental pollution. Keratinase enzymes that degrade keratin may be used in poultry waste bioremediation. The objectives were to screen keratinolytic isolates, identify them, optimize culture conditions, and measure activity.
Methods: Two keratinolytic isolates were screened from poultry waste around Mashhad, Iran. They were identified, culture conditions were optimized, and activity was measured using azokeratin and turbidity absorbance.
Results: The two isolates were identified as Bacillus pumilus and Bacillus tequilensis. The optimal conditions were pH 7.0, 37°C, and 48 h of incubation. The maximum keratinase activity was 120 U/mL and 100 U/mL, respectively.
Conclusion: The two Bacillus isolates showed potential for poultry waste bioremediation and keratinase production applicable to animal feed and cleaning products.

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