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Abstract Background and objectives: Ureaplasma urealyticum is one of the most important causes of Nongonococcal urethritis (NGU) and non-specific urethritis (NSU) in men. Also Mycoplasma hominis has a role in causing NGU and NSU. The aim of this study was the detection of Mycoplasma hominis & Ureaplasma urealyticum in men suffering from NGU and NSU by prostatic massage method and first- void Urine samples. Material and Methods: Urethral secretion samples, taken after prostatic massage and first-void urine, of 75 patients referred to department of bacteriology of Tehran University of Medical Sciences were examined for ureaplasma and mycoplasma by culture method in 2004-2005 Results: In prostatic samples, 19 and 11 samples are positive for ureaplasma and Mycoplasma, retrospectively. In first-void urine samples, 17 and 9 samples are positive for ureaplasma and Mycoplasma, retrospectively. Conclusion: The results show that if it is impossible to obtain urethral sample via prostatic massage , we can use first- void urine instead. Key words:Mycoplasma hominis, Ureaplasma urealyticum,Nongonococcal urethritis, Nonspecific urethritis, prostatic massage, first voided Urine

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Abstract Background and objectives: Infection has a Leading role in pregnancy. Cytomegalovirus (CMV), listeria and Toxoplasma are the most common causes of infection in human. Based on the previous researches, about 15-25 percent of being infected during pregnancy leads to some complications such as abortion, fetal death, early labor and etc. This study was designed to determine the seroprevalence of Cytomegalovirus (CMV), Toxoplasma gondii and Listeria moncytogenes among pregnant women in Gorgan, north of Iran (2005-2006). Material and Methods: we conducted this Simple randomized study on 118 unsuccessful pregnant woman and 99 successful ones referred to Deziani hospital in Gorgan. We assayed both IgG and IgM antibodies for CMV and Toxo by Elisa and IFA method for Listeria. In addition, we fill out a Check list and then use SPSS soft ware, chi square to analyze the data. Results: The frequency of IgG for CMV and Toxo is 89.9% and 45.5% in successful pregnant women and 77.1% and 44.1% for unsuccessful pregnant women (P=0.41, P=0.01). IgM frequency for CMV and Toxo is 14.1% and 46.5% in successful women and 30.5 and 21.7% in unsuccessful ones. (P=0.003, P=0.002)Total frequency (IgG, IgM) for Listeria is 7.62% and %3.03 in successful and unsuccessful women, respectively. There is a significant relation between abortion and IgM titer against Toxoplasma in successful and unsuccessful groups. (P=0.003).This relation is true for total antibody titer against Listeria (P=0.003). Conclusion: Because of high titer of antibodies against CMV, Toxo and Listeria in unsuccessful pregnant women, suffering from these agents during pregnancy may result in abortion and fetal death. Hence, we recommend to hold some preventive and educational program and also to assay antibodies against theses agents. Key words: Listeria moncytogenes, Cytomegalovirus (CMV), Toxoplasma gondii, success and non-success pregnancy, Serology, Gorgan

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Gestational diabetes mellitus (GDM) is the most common metabolic disorder during pregnancy which is associated with 3 to 4 folds of increase in the risk of maternal and fetal morbidity, if it is not diagnosed early (1). Gestational diabetes occurs from 1% to 14%, and 90% of pregnancy-related diabetes has been related to gestational diabetes (2). Glucose challenge test (GCT) and glucose tolerance test (GTT) are recommended by American Diabetes Association (ADA) for screening and diagnosing of gestational diabetes (3). In this cross-sectional study, the subjects were the pregnant women during 24-28 weeks of gestation referred to Khatam-o-allanbia laboratory of Arak city in 2009. First, we explained the aim of the project and then obtained the written consent. After being fast for at least 8-12 hours, five ml of their venous blood was drown. For GCT, the blood samples were obtained one hour after consumption of 50g of glucose powder. The Individuals whose GCT was equal or higher than 130 mg/dl were introduced for GTT in the future days. After 8-12 hours of being fast, blood samples were taken to perform GTT. Then after eating 100 grams of glucose powder, their 1, 2 and 3 hour -venous blood samples were drown to perform glucose test, using glucose testing kit of Pars Azmun Company and Mindary BS-300 autoanalyser. The average age of referred pregnant women is 27.37±10.24 year. Of 417, 403 (96.64%) have normal fasting plasma glucose (≤ 99 mg/dl) and 14 (3.36%) have abnormal fasting plasma glucose (≥ 100 mg/dl). When normal GCT is considered ≥140 mg/dl and ≥130 mg/dl, 357 (85.61%) and 308 (73.56%) have normal GCT and 60 (14.39%) and 109 (26.14%) have abnormal GCT, respectively. The Average of fasting plasma glucose (FPG) and GCT are 83.86 (73-243) and 118.32 (140-351) mg/dl, respectively. In 9 (64.29%) of women with abnormal FPG, the GCT test is also abnormal. By performing GTT for the women whose GCT was equal or higher than 130 mg/dl, 24 (5.76%) were identified with gestational diabetes and introduced for treatment. If 140 mg/dl was considered as a cutoff point of GCT, four women (0.94%) would falsely be non-diabetic. Based on this study, the prevalence of GDM is 5.74% that is not so high, compared to the range of 4.8 to 7.4% of the country (4). A study conducted by Soheilykhah et al. on 1071 pregnant women with GCT of ≥ 130 mg /dl, 31.9% of the women had abnormal GCT. After doing GTT, 110 patients (10.2%) have been diagnosed with gestational diabetes (2) that is higher than the prevalence of other studies in Iran. Due to results of this study and other studies, it seems that the GCT is not a reliable test for screening gestational diabetes. On the other hand, if GCT is going to be used, further studies are required to review the current Cutoff point of this test. Key words: Glucose challenge test (GCT), Fasting plasma glucose (FPG), Gestational diabetes.

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Abstract Background and Objective: Toxoplasmosis is one of the most prevalent parasitic infections worldwide. Contamination of pregnant women with toxoplasmosis may cause fetal death, preterm delivery and congenital toxoplasmosis. Due to importance of congenital Toxoplasmosis and the need of further study, this research was accomplished in Zahedan. Material and Methods: The serum samples (N= 221) were collected from pregnant women referring to reference laboratory of Zahedan in 2011. The IgG and IgM antibody levels against toxoplasmosis were investigated using ELISA method. Results: Out of all samples, 30.8% are IgG positive and 1.4% are both IgG and IgM positive. There is no significant difference between positive and negative groups using Chi-square tests. Conclusion: The main part of pregnant women in Zahedan (69.2%) is serologically negative against toxoplasmosis therefore, hygiene education to eliminate risk factors especially during pregnancy period seems to be imperative. Keywords: ELISA Antibody Pregnancy Toxoplasma Zahedan

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Abstract Background and Objective: Mycoplasma hominis and Ureaplasma urealyticum are recognized as the main pathogens of urogenital tract. Mycoplasma genitalium can cause several reproductive tract inflammatory syndromes in women. Material and Methods: This study was conducted on 100 women with the history of abortion. The samples were obtained from cervical secretions and remaining products of curettage to diagnose Genital Mycoplasma. All samples were inoculated into PPLO Broth medium and incubated at 37ºC for 3 days. Results: Of 21 Mycoplasma isolates, Mycoplasma hominis was isolated from both cervical secretions and remaining products of curettage (4.26%) Ureaplasma urealyticum was isolated from products of curettage (12.8%), cervical secretions (8.5%) and from both samples (6.38%). Conclusion: Based on the results, a high percentage of women with the history of abortion were infected with these bacteria. Thus, early diagnosis and treatment of these pathogenic organisms are necessary. Key words: Mycoplasma Hominis Ureaplasma Urealyticum Abortion

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ABSTRACT
        Background and Objectives: Infertility is one of the important causes of anxiety in couples. Infections caused by genital Mycoplasmas may have harmful effects on the reproductive health of women, and sometimes lead to infertility. This study was designed to determine frequency of anti-Mycoplasma hominis antibodies in infertile women at Al-Zahra Hospital, Tabriz, Iran.
       Methods: In this cross-sectional study, serum from 184 infertile women and 100 healthy pregnant women were tested for presence of M. hominis IgM and IgG antibodies by ELISA. Data collected were analyzed in SPSS (version 17) using t-test at significance level of 0.05.
         Results: The frequency of anti-M. hominis IgG was significantly higher in infertile women compared to healthy controls. The frequency of anti-M. hominis IgM did not differ significantly between the infertile and control subjects. The majority of the women infected with the bacterium were in the 21-30 and 31-40 years age range. There was no significant correlation between tubal factor infertility and seropositivity for M. hominis antibodies. Moreover, the dwelling of the participants had no relationship with the frequency of anti-M. hominis antibodies. 
        Conclusion: The frequency of owed that a high percentage of t a high percentage of that the meaninngful le women was 4% and infertile women infected with this bacterium is high in Tabriz. Therefore, it is important to perform microbial screening for this bacterium in infertile couples. 
       Keywords: Mycoplasma hominis, Infertility, Women, Tabriz.
ABSTRACT
        Background and Objectives: Infertility is one of the important causes of anxiety in couples. Infections caused by genital Mycoplasmas may have harmful effects on the reproductive health of women, and sometimes lead to infertility. This study was designed to determine frequency of anti-Mycoplasma hominis antibodies in infertile women at Al-Zahra Hospital, Tabriz, Iran.
       Methods: In this cross-sectional study, serum from 184 infertile women and 100 healthy pregnant women were tested for presence of M. hominis IgM and IgG antibodies by ELISA. Data collected were analyzed in SPSS (version 17) using t-test at significance level of 0.05.
         Results: The frequency of anti-M. hominis IgG was significantly higher in infertile women compared to healthy controls. The frequency of anti-M. hominis IgM did not differ significantly between the infertile and control subjects. The majority of the women infected with the bacterium were in the 21-30 and 31-40 years age range. There was no significant correlation between tubal factor infertility and seropositivity for M. hominis antibodies. Moreover, the dwelling of the participants had no relationship with the frequency of anti-M. hominis antibodies. 
        Conclusion: The frequency of owed that a high percentage of t a high percentage of that the meaninngful le women was 4% and infertile women infected with this bacterium is high in Tabriz. Therefore, it is important to perform microbial screening for this bacterium in infertile couples. 
       Keywords: Mycoplasma hominis, Infertility, Women, Tabriz.

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Background and Objectives: Reduced insulin secretion in Type II Diabetes Mellitus (T2DM) results in fat accumulation in hepatocytes and increase in liver enzymes. The hepatocyte destruction due to non-alcoholic fatty liver is associated with increased risk of cardiovascular diseases. Therefore, the aim of this study is to evaluate the association of liver transaminases with lipid profile in T2DM patients compared to healthy subjects.
Methods: A total of 1000 subjects (500 males and females with T2DM and 500 healthy subjects) participated in this case-control study. Diagnosis of T2DM was made based on the World Health Organization (WHO) criteria. Fasting blood samples (5 ml) were obtained from all subjects in the morning and the serum was extracted for the assessment of biochemical parameters was used to compare parameters between healthy and T2DM patients. Data were analyzed by SPSS version 20 and using Mann-Whitney test and Spearman correlation coefficient.
Results: The serum total cholesterol (TCH), triglyceride, Alanine Aminotransferase (ALT), Aspartate Transaminase (AST), TCH/high density lipoprotein (HDL-C) ratio and atherogenecity index plasma were significantly higher in T2DM group compared to healthy individuals (p≤0.006). Among the lipid profile parameters only Risk ratio (TCH/HDL-C) was positively correlated with ALT (p=0.037) while no significant correlation was observed for other variables (P>0.05)
Conclusion: T2DM Patients have higher lipid profile, atherogenecity index and liver enzymes compared to healthy individuals. Furthermore, TCH/HDL-C was significantly correlated with ALT.

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Plasma cell leukemia (PCL) is a rare form of plasma cell dyscrasia with 2 variants: the primary form, which occurs de novo in patients with no previous history of multiple myeloma (MM), and the secondary form, which represents a leukemic transformation in patients with a previously recognized MM. Unlike myeloma, PCL typically follows an aggressive course, and the median age at presentation is usually above 50 years. In this report, we present a case of primary PCL that manifested at 19, an exceptionally rare occurrence.
 

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Multiple myeloma (MM) is a plasma cell neoplasm that is characterized by the clonal proliferation of malignant plasma cells in the bone marrow. It is considered the second most common hematological malignancy which accounts for approximately 1% - 2% of all cancers and among 10% of hematological malignancies. Autologous peripheral blood stem cell Transplantation (PBSCT) is the best treatment for MM. Since the optimum harvested stem cell yield is a crucial factor for sufficient engraftment, the enumeration of Mononuclear cell (MNC) count in peripheral blood and harvested CD 34+ stem cell count can be considered as the best predictive markers for the best timing of apheresis which positively correlates with engraftment outcome of PBSCT.
MNC count can be obtained using either a hematological analyzer or peripheral blood smear while flow cytometry is the advanced technology that can be used to enumerate CD 34+ stem cell count other than peripheral blood smear. The unavailability of a flow cytometer, the expensiveness of this method, and the lack of trained personnel regarding this new technology, especially in lower-middle-income countries cause disturbance in the enumeration of stem cells. In such a situation, this review describes the importance of establishing an association between peripheral blood MNCs and harvested CD 34+ cells. Furthermore, this association facilitates conducting effective PBSCT for MM patients even in the absence of a flow cytometer and eventually, it focuses on decentralizing the treatment of PBSCT.

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Background: Blood products are vital yet scarce medical resources. In teaching hospitals, inefficient transfusion practices often result in unnecessary waste, compromising both patient safety and healthcare costs. This study investigated the patterns and underlying causes of unused blood products at Imam Reza Hospital, a major tertiary center in northeast Iran, with the aim of delineating modifiable factors contributing to this issue.

Methods: This retrospective cross-sectional study was conducted at Imam Reza Hospital, Mashhad. Data were extracted from the Laboratory Information System (LIS) and the hospital’s dashboard for the period of March 2022–2025. Blood component reserve and return data for five large clinical departments were examined.

Results: Although the total number of returned units declined over time, the return percentage increased in the final year, indicating persisting inefficiencies. By department, Neurology and Gynecology exhibited the highest return percentages, while the Hematology-Oncology ward, Intensive Care Unit (ICU), and Emergency Department reported the highest absolute numbers of returns. Platelets were the most frequently returned product, whereas fresh frozen plasma had the highest relative return rate. The most consistently reported reason for returns was “Physician Decision / Order Canceled,” with returns due to “Patient Death” also showing a gradual increase over the study period.
Conclusion: Despite gradual improvements, systemic challenges—especially poor documentation, absence of digital monitoring tools, and knowledge gaps in transfusion handling—continue to undermine efficient blood product use. Implementing real-time dashboards, enforcing mandatory documentation, educating clinical staff, and adopting AI-based demand prediction tools could dramatically reduce waste and enhance transfusion practices.