Sepideh Yamini, Zohre Eftekhari, Mohammad Reza Mokhber Dezfouli, Babak Beikzadeh, Parvane Mehrbod,
Volume 13, Issue 6 (11-2019)
Abstract
ABSTRACT
Background and Objectives: Exogenous
lung surfactant (ELS) obtained from extraction of bronchoalveolar lavage fluid, is prescribed in some respiratory disorders, which could affect production of lung-related cytokines. Interleukin-8 (IL-8) is a major cytokine that could affect severity of lung diseases. In this study, we investigate the possible impact of ELS on IL-8 expression, hematological parameters and IgG and IgM levels in rabbits.
Methods: ELS solution obtained from fresh calf’s lung bronchoalveolar lavage was infused into the lungs of five rabbits via tracheal tube. Blood samples were collected before and after ELS treatment for 30 days.
Results: Serum IL-8 levels decreased over time following ELS administration. IL-8 expression also decreased after exposure to ELS, but leukocytes count increased significantly 24, 48 and 72 hours after ELS therapy compared to baseline values (P<0.05). IgM level increased significantly 72 hours after the ELS therapy and returned to normal range at the end of study.
Conclusion: Our findings suggest that ELS could down-regulate IL-8 expression in mononuclear cells while increasing leukocytes population and total immunoglobulins level, which can trigger immune responses without lung damage. However, further studies should be performed to confirm the regulatory effects of ELS on inflammatory responses in lung diseases.
Keywords: Exogenous lung surfactant, IL-8, Immunoglobulins
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Dr Dipankar Thakuria, Dr Karan Kumar Borah, Dr Projnan Saikia, Dr Kusum Borsaikia,
Volume 19, Issue 3 (7-2025)
Abstract
Background: Pathological autopsy plays a crucial role in understanding mortality causes, especially in cases where diagnosis during life was incomplete or impossible. The collaboration between forensic experts and pathologists provides valuable insights into the cause and manner of death. Aim: The study aimed to determine the prevalence and pattern of lung, liver, and kidney histopathology, assess demographic associations, and investigate etiological factors contributing to these pathologies. Methods: A retrospective, non-interventional, record based cross sectional study was conducted for a period of five years from 1st January 2019 to 31st December 2023, analyzing 90 autopsy cases excluding decomposed bodies and cases primarily due to extensive trauma. Results: Lung pathology was observed in 61.1% of cases, predominantly showing congestion and inflammation. Liver diseases were found in 47.7% of cases, with steatosis and cirrhosis notable, especially among males. Kidney pathology was present in 50% of cases, with various changes noted. Demographic distribution indicated a higher prevalence among males, with sudden death, chronic alcohol abuse, and road traffic accidents being common causes. Conclusion: The study emphasizes the significant burden of lung, liver, and kidney pathology in autopsy cases, particularly among males, highlighting the importance of autopsy in understanding the underlying causes of death and guiding public health interventions and forensic investigations.
