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Showing 2 results for Interferon-Gamma
The effect of nettle extract consumption and endurance training on the expression of IFN-γ and Endostatin in the liver tissues of mice with melanoma
Vahid Zolghadri , Alireza Barari , Asieh Abbasi Daloii , Hosein Abed Natanzi ,
Volume 19, Issue 5 (9-2025)
Abstract
Background: Melanoma is an aggressive malignancy that results from the transformation of pigment-producing melanocytes. This study aimed to investigate the effect of nettle extract consumption and aerobic exercise on the gene expression of IFN-γ and Endostatin in the liver tissues of mice with melanoma.
Methods: Twenty male BALB/c mice were randomly divided into four groups, including control, endurance, nettle, and endurance+nettle. The training program included running on a treadmill for 30 minutes at a speed of 16 meters per minute. The speed was increased by one meter per minute each week, reaching 22 meters per minute in the eighth week. Melanoma cells were injected subcutaneously into the left side of the mice. The experimental groups received 30 mg/kg/day of nettle ethanol extract orally for eight weeks. Real-time PCR was used to assess the expression of IFN-γ and Endostatin.
Results: IFN-γ expression levels in the experimental groups were not different from the control group, while Endostatin levels were significantly reduced (p = 0.142, p <0.001, respectively). IFN-γ expression levels in the experimental groups were higher than in the control group, but did not reach a significant level. Also, Endostatin expression levels in training and combination groups were significantly lower than in the control group (p = 0.022, p <0.001, respectively).
Conclusion: The results showed that endurance training combined with nettle extract may inhibit angiogenesis and capillary tissue formation in the tumor tissue of mice with melanoma by increasing IFN-γ and decreasing Endostatin.

Effect of interferon- on the expression of regulatory NLRP6 and NLRP12 genes in human Wharton’s jelly mesenchymal stem cells
Leila Pirdel, Maryam Safajoo, Masoud Maleki,
Volume 19, Issue 5 (9-2025)
Abstract
Background: Mesenchymal stem cells (MSCs) are well-known for their immune-modulatory properties. A subgroup of the nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) has been recently identified to play a regulatory role in immune and inflammatory responses. This study aims to analyze and compare the gene expression levels of the NOD-like receptor family pyrin domain-containing proteins (NLRPs), such as NLRP6 and NLRP12, in Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) treated with interferon-gamma (IFN-γ), the pro-inflammatory cytokine, and untreated cells.
Methods: The immunophenotypic characterization of the isolated Wharton’s Jelly mesenchymal stem cells (WJ-MSCs) was conducted using flow cytometry. Next, they were cultured with or without IFN-γ, followed by a comparison of the expression levels of the NLRP6 and NLRP12 genes using quantitative PCR (qPCR).
Results: Treatment of cells with IFN-γ resulted in a statistically significant increase in NLRP12 gene expression compared to untreated cells. In contrast, the expression of NLRP6 did not differ significantly between cells with or without IFN-γ treatment.
Conclusion: The altered expression level of NLRP12 suggests its potential role in the inflammatory regulation mediated by WJ-MSCs in response to IFN exposure; however, further studies are needed to validate its role in experimental models of inflammatory-related diseases.

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