Showing 17 results for Extract
A Alavi, Sh Moradi, N Mirkheshti, A Ghadiri, F Hadizadeh,
Volume 1, Issue 1 (4-2007)
Abstract
Abstract Background and objectives: Hemin is a porphyrin compound derived from hemoglobin, the precursor of other porphyrin hemoglobin derivatives and the raw material of Hematin. Since hemin is widely used in medicine, we decided primarily to synthesize this substance in Laboratory and to determine the best way of hemin extraction from untransfused and expired blood units. Materials and Methods: In the first method, Glacial acetic acid and sodium chloride were added to citrated blood and hemin crystals were extracted by means of cooling. Finally, the obtained product, by visible spectrophotometer and Infrared Spectrophotometer, was compared to standard samples. Fur thermore, citrated blood, citrated blood hemolysed by distilled water and citrated blood washed by normal saline were used comparatively as a raw material to produce Hemin. The second method was performed by adding Strontium, acetic acid and acetone to blood samples and then after precipitating Hemin crystals they were washed and dried with acetone. Results: The presence of functional groups in Hemin samples, analyzed by infrared Spectrophotometer, indicates the production of this compound. The results of visible Spectrophotometer in comparison with control samples and the results of samples weighting demonstrates high efficiency of extraction stages and the purity of obtained compound. Conclusion: The use of intact citrated blood produces more Hemin than the other kind of Citrated blood samples. Moreover, acetic acid with citrated blood, without any processing on blood, is the best way for Hemin production. Key words: strontium, Hemin, Blood, acetic acid, extraction
H Davoodi, S R Hashemi, H F Seow, M Ghorbani,
Volume 3, Issue 2 (10-2009)
Abstract
Abstract Background and objectives: Paraffin-embedded tissues and clinical samples are a valuable resource for molecular genetic studies, but the extraction of high-quality genomic DNA from this tissues is still a problematic issue. In the Present study, the efficiency of two DNA extraction protocols, a commercial kit and a traditional method based on heating and K Proteinase was compared. Material and Methods: Fifty paraffin-embedded blocks of colon cancer tissues (more than 5 years old) were used to compare two methods of DNA extraction. DNA was extracted by traditional method using heat and commercial DNA extraction (Qiagen kit) method. Then the purity and concentration of extracted DNA were measured by Spectrophotometer. Two sequences of TLR4 “The most important receptors in innate immunity” were amplified by polymerase chain reaction. SH-1 ‘188bp’ and SH-2 ‘124bp’ were amplified and then the products were separated on a 2% agarose gel. Results: The results show that the yield of DNA by traditional method (297 mg/ml) is significantly (p<0.01) higher than Commercial kit (176mg/ ml). But traditional method has the lower OD ratio (1.2) Compared to Commercial method. The Amplification of the TLR4 gene sequences is more successful by the traditional method (p<0.01) compared with commercial method. The length of the sequence affects on the results of PCR in that short sequence is amplified more successful compared to the long sequence. Conclusion: The traditional method is more successful in PCR amplification and also simple and cheap. Therefore, we recommend using this method for DNA extraction taken from the paraffin-embedded blocks with more than 5 years old and selecting shorter sequence for better amplification in PCR. Key words: DNA Extraction, paraffin embedded tissue, PCR
Mousazade Moghadam M, Babavalian H, Mirnejad R, Shakeri F,
Volume 6, Issue 1 (4-2012)
Abstract
Abstract
Background and objectives: Genomic DNA extraction of bacterial cells is of processes performed normally in most biological laboratories therefore, various methods have been offered, manually and kit, which may be time consuming and costly. In this paper, genomic DNA extraction of Staphylococcus aureus was investigated using some laundry detergent brands available in Iran to achieve a rapid and cost effective method.
Material and Methods: five-enzyme Taj brand, three-enzyme Saftlan brand ,and Darya and Pak brands without enzyme were used in the concentrations of 10, 20, 40, 80 mg/L. Afterwards, in order to evaluate the efficiency of extracted DNA in downstream processing, PCR test was performed for femA gene in the genome of Staphylococcus aureus.
Results: DNA extraction using different concentrations of the brands show that extracted DNA using 40 mg/L Saftlan and Taj brand powders have the best results according concentration (µg/ml) and purity (A260/A280) parameters. These parameters are 387.5 1.88 (Taj), 254.1 2.80 (Softlan), 396.6 1.95 (Manual) and 423.3 2.2 (Kit), respectively. Afterward, the PCR test results by show that DNA extraction using laundry detergents has no effect on its efficiency in order to be used in downstream processes.
Conclusion: These results indicate that the proper concentrations of laundry detergents can be used to extract genomic DNA with similar efficiency to kit and manual extraction methods.
Key words: Bacterial genome, DNA extraction, laundry powder, PCR, Staphylococcus aureus
M Raeisi, H Tajik, J Aliakbarlu,
Volume 6, Issue 2 (10-2012)
Abstract
Abstract
Background and objectives: The presence of pathogenic bacteria and the factors causing food spoilage are the great challenge for public health. Attention to natural additives instead of chemical preservatives resulted in conducting several studies on plant essential oil and extracts. We aimed at evaluating the antibacterial effect of carboxymethyl cellulose coating enriched by Zataria multiflora essential oil and grape seed extract on rainbow trout meat.
Material and methods: In this study, two concentrations of Zataria multiflora essential oil (1% and 2%) and two concentrations of grape seed extract (0.5% and 1%) were used both alone and in combination with Carboxymethyl cellulose coating. Antibacterial effect of these treatments was evaluated by enumeration of bacteria in special culture media.
Results: The results obtained in this study demonstrate that Zataria multiflora essential oil in combination with grape seed extract significantly can decrease the number of bacteria and delay the spoilage of the samples (p<0.05).
Conclusion: Coating enriched by Zataria multiflora and grape seed extract can properly delay the growth of spoilage microorganisms and prolong the shelf life of meat products.
Key words: Carboxymethyl cellulose coating, Zataria multiflora essential oil, Grape seed extract, Microbial flora
S Forouzandeh, N Naghsh, S Salimi, D Jahantigh,
Volume 8, Issue 1 (4-2014)
Abstract
Abstract
Background and Objective: Cervical cancer is the second most common cancer in women. Boswellia serrata is a medicinal herb with anticancer, antibacterial, antiulcer, antifungal properties. Since the antitumor effect of this medicine has not been studied on cancer cell lines, we aimed to investigate the antitumor effect of Boswellia serrata on cervical cancer cell lines.
Material and Methods: To assess the anti-cancer effect of Boswellia serrata extract, HeLa cell lines were cultured , propagated and placed with different doses of Boswellia serrata (12.5,25, 50 and100 µg/ml) for 24,48and72 hours. After that, MTT test was used to determine the cellular toxicity of the extract.
Results: The results of the MTT test showed that this extract has dose-dependent and time-dependent anti cancer effect on Hela in that the highest effect was seen with 100 µg/ml of extract for 72 hrs. The half maximal inhibitory concentration (IC50) for 24 and 48 hrs were 12.5 and 50 µg/ml, respectively. In 72 hours, due to increase of incubation period in all concentrations, the number of killed cells was more than 50 percent. Consequently, IC50 was not observed for this period of time.
Conclusion: Considering dose-dependent and time-dependent anti cancer effect, Boswellia serrata extract can inhibit the growth of Hela cells.
Keywords: Hela Cell MTT Test Boswellia Serrata Extract Cervical Cancer
M Rajabi, R Habibipour, S Vesaghati Ezatpour, S Vesaghati Ezatpour,
Volume 8, Issue 3 (8-2014)
Abstract
Abstract
Background and objective: Antimicrobial and antiviral effects of Alloe Vera and Stachys inflata have been proved. We aimed to investigate the effects of extract of Alloe Vera and Stachys inflate on the growth of some bacteria to take the place of chemical drugs.
Material and Methods: the extracts of both plants were prepared by maceration method different concentrations were prepared using Mueller Hinton agar medium and tested by Disc diffusion. Furthermore, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by the Microdilution method.
Results: The effect of Alloe Vera extract was significant on Staphylococcus aureus. MIC and MBC of Aloe Vera extract on Bacillus subtilis were obtained in 230 and 410 mg/ml, respectively, which were 500 and 714 mg/ml for Haemophilus influenza. The extract of gel of Alloe Vera had no effect on Bacillus subtilis. The extract of leaf and gel of Alloe Vera had an inhibitory effect on Haemophilus influenza and Pseudomonads aeraginosa. The extract of Stachys inflata had an inhibitory effect on Haemophilus influenza, but it did not have any on Pseudomonads aeraginosa. The Extract of Stachys inflata had no effect on Bacillus subtilis, while showing significant effect on Staphylococcus. Among antibiotics, Ofloxacin had an effect on Haemophilus influenza. The extract of both plants did not show any effect on Klebsiella pneumonia.
Conclusion: Given the effect of Alloe Vera and Stachys inflata in laboratory conditions, we hope that these extracts will be used instead of chemical substances for making nutritional supplements to control human diseases.
Key words: Alloe Vera, Antibacterial, Stachys Inflata, Extract
Shaghayegh Nikpour Moghadam , Shokoufeh Nikpour Moghadam ,
Volume 10, Issue 1 (1-2016)
Abstract
Abstract
Background and Objective: Enterococci are relatively nonvirulent bacteria that rarely cause disease. Antimicrobial treatment of Enterococci is often challenging due to their antibiotic resistance. This study aimed to investigate the antibacterial activity of aqueous extract of garlic against Enterococcal isolates.
Methods: In this descriptive study, 120 Enterococcus isolates including 70 multidrug-resistant isolates were collected from hospitals of Babol, Iran. Isolates’ susceptibility to different antibiotics and the antibacterial activity of garlic extract were assessed using methods of minimum inhibitory concentration (MIC) measurement. The experiments were performed according to the Clinical & Laboratory Standards Institute guidelines, using Tryptic soy broth medium and disc diffusion method.
Results: Among the 120 Enterococcal isolates, 95 (79.2%) and 25 isolates (20.8%) were E. faecalis and E. faecium, respectively. Of the all Enterococcal isolates, the highest resistance was to erythromycin (95.8%), tetracycline (88.3%) and ampicillin (65.8%). While, the minimal level of resistance was to chloramphenicol (6.8%), vancomycin (20%) and ciprofloxacin (25%). Also, 53.3% of Enterococcal isolates showed simultaneous resistance to at least three antibiotics (tetracycline, erythromycin and ampicillin). Such resistance in E. faecium isolates was higher compared to E.faecalis (68% vs. 55.7%). The range of antibacterial activity of garlic extract against isolated Enterococci was determined by growth inhibition zone of 16.8 ± 1.8 mm and MIC of between 4 to 32 mg/ml.
Conclusion: This study indicates the clear anti-enterococcal effect of aqueous extract of garlic and confirms the use of garlic in treatments by medicinal plants.
Seyedeh Masoumeh Mirtaghi, Parisa Torbati Nejad , Masoumeh Mazandarani, Fasiheh Livani, Hanieh Bagheri ,
Volume 10, Issue 5 (9-2016)
Abstract
Background and Objective: Nowadays, incidence of antibiotic-resistance among pathogenic bacteria has increased due to indiscriminate use of antimicrobial drugs for treatment of diseases, especially urinary tract infections. Medicinal plants are also of great importance as antibacterial agents. Therefore, the aim of this study was to determine the antibacterial effect of ethanolic extract of nettle (Urtica dioica L.) leaves using two methods of disk diffusion and well diffusion.
Methods: Ethanolic extract of nettle leaves was prepared by the percolation method. Effect of different concentrations of the extract on Escherichia coli (PTCC1399), Staphylococcus aureus (PTCC 1431), Staphylococcus epidermidis (PTCC 1435) and Staphylococcus saprophyticus (PTCC1440) was evaluated using the disk diffusion and well diffusion methods by measuring diameter of growth inhibition zone. Gentamicin and propylene glycol were used as positive and negative control, respectively.
Results: In both methods, especially in the well diffusion, the ethanolic extract of nettle leaves had favorable inhibitory effect on the growth of S. aureus, S. epidermidis and S. saprophyticus. In the well diffusion method, the highest rate of susceptibility to the extract (89%) was related to S. saprophyticus and S. epidermidis.
Conclusion: The ethanolic extract of nettle leaf has good inhibitory effect on the growth of S. aureus (especially in the well diffusion method), which confirms the traditional use of this plant for the treatment of urinary tract infections.
Keywords: Antibacterial Effect, Staphylococcus, E. Coli, Ethanolic Extract, Nettle (Urtica dioica L.).
Saeid Farshbaf Derhami , Mehdi Ghiami Rad , Razzagh Mahmoudi,
Volume 10, Issue 6 (11-2016)
Abstract
ABSTRACT
Background and Objective: Although antibiotics are commonly used for treatment of infectious diseases, these treatments are often associated with several problems such as unwanted side effects and resistance to antibiotics. The aim of this study was to evaluate the antibacterial effect of aqueous and alcoholic extracts of Nasturtium officinale on Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes.
Methods: All experiments were performed using the well diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the aqueous and alcoholic extracts of N. officinale against the pathogenic bacteria studied were determined by microdilution method.
Results: In the well diffusion method, S. aureus and L. monocytogenes were the most sensitive bacteria with MIC of 8 μg/ml, while E. coli and S. typhimurium were the most resistant bacteria to the aqueous and alcoholic extracts. In addition, the inhibitory activity of the alcoholic and aqueous extracts of N. officinale was higher against gram-positive bacteria compared to gram-negatives. The lowest MIC (6.25 μg/ml) and MBC (12.5 μg/ml) of the plant extract were against S. aureus.
Conclusion: The aqueous and alcoholic extracts of N. officinale affect the growth of gram-positive bacteria (S. aureus and L. monocytogenes) but not the gram-negatives (S. typhimurium and E. coli). These extract could be used for treatment of infections.
Keywords: Nasturtium Officinale, Aqueous Extract, Alcoholic Extract, Antibacterial Effect.
Younes Anzabi, Arash Khaki,
Volume 10, Issue 6 (11-2016)
Abstract
ABSTRACT
Background and Objectives: Many species of Ziziphora have been used in traditional medicine in the Azarbaijan region, northwest of Iran. This study aimed to determine antibacterial activity of essential oil and methanol extract of Ziziphora tenuior on some pathogenic bacteria isolated from urogenital tract infections.
Methods: The essential oil and methanol extract of Z. tenuior were obtained by Clevenger and maceration methods. Under sterile conditions, the required amount of urine was taken from patients referred to a number of clinics in Tabriz during 2014. After identification of the isolates using standard microbiological methods, antimicrobial effects of the essential oil and methanol extract of the plant on the isolates were evaluated by determining minimum inhibitory concentration (MIC), minimum bactericidal concentration, and antibiogram test.
Results: The MIC for essential oil of Z. tenuior was 250µg/mL for most Gram-negative bacteria except Pseudomonas aeruginosa. Moreover, the MIC for Staphylococcus aureus was 250µg/mL and 500µg/mL for other strains of Staphylococcus.
Conclusion: Comparison of the inhibitory and bactericidal effects of the essential oil and methanol extract of Z. tenuior showed that the essential oil is able to inhibit growth of the bacteria tested even in low concentrations. Further studies are required in this regard using animal models.
Keywords: Antibacterial Agents, Ziziphora tenuior, Plant Extracts, Urinary Tract Infection.
Jaber Ali Poor , Mansor Dabirzadeh, Yahya Marofi , Seyed Ali Asghar Sefidgar,
Volume 11, Issue 3 (5-2017)
Abstract
ABSTRACT
Background and objective: Surgery is the gold standard treatment for hydatidosis. Scolicidal agents could be used during surgery to kill
protoscoleces and prevent cyst recurrence after rupturing of main lesion. Non-chemical agents with sufficient
protoscolex-killing activity are known to be fully effective in this regard with fewer side effects. Fig tree is an Iranian native plant, which has been used for medicinal purposes in traditional medicine.
Methods: After obtaining infected hydatid cyst of the liver from a slaughterhouse in Babol (Iran), the percentage of live
protoscoleces was calculated by critical staining with 0.1 % eosin. Then, the protoscolex-killing activity of methanolic extract of fig leaves was evaluated at concentrations of 2.5, 5, 10, 15 and 20 mg/ml in 2, 4, 8, 12, and 16 minutes exposure times
. Statistical analysis was performed using SPSS software (version 22).
Results: Concentration of 20 mg/ml of the extract showed 100% protoscolex-killing activity within the first 2 minutes of exposure. In addition, the lowest protoscolex-killing activity (45%) was observed at concentration of 2.5 mg/ml after 2 minutes. The results also showed that the protoscolex-killing activity of the extract increases significantly in a concentration- and time-dependent manner (P <0.001).
Conclusion: The methanolic extract of fig leaf at concentration of 20 mg/ml could exert significant scolicidal effect within 2 minutes of exposure. Therefore, complementary experiments should be performed on animal models to further assess the efficiency of the extract for killing protoscoleces of hydatid cyst during surgery.
Keywords: Echinococcus granulosus, methanolic extract of
Ficus carica, broth dilution.
Maryam Meskini, Mohsen Korani , Davoud Esmaeili ,
Volume 13, Issue 4 (7-2019)
Abstract
Background and Objectives: Burns are a major global public health problem, accounting for an estimated 180,000 deaths annually. The majority of burn-related deaths occur in low- and middle-income countries. Considering the importance of treating infected burn wounds with the least adverse effects, we aimed to search the literature to find new treatments for infected wounds using medicinal plants.
Methods: The search process was carried out using various databases including Google Scholar, ScienceDirect, Web of Science, MEDLINE, PubMed, Scopus, and the Cochrane Library. We searched for relevant original and review articles (published in English or Persian) using the following keywords: herbal extract, herbal medicine, burn infection, and wound infection.
Results: Overall, we found approximately 100 articles related to the use of medicinal plants for treatment of wounds or infections. According to these studies, main constituents of plant extracts were carvacrol, flavonoids, terpenoids, phenolic diterpenes, and phenolic acids. Most studies assessed the antimicrobial activity by determining minimum inhibitory concentration and minimum bactericidal concentration using the disc diffusion method.
Conclusion: Given the favorable antimicrobial activity of medicinal plants, it is recommended to use them for treatment of burn wound infections.
Fereshteh Keyghobadi, Nader Bahramifar, Elahe Gharekhani, Seyyedeh Marzieh Kia,
Volume 13, Issue 5 (9-2019)
Abstract
ABSTRACT
Background and Objectives: In this study, nanosilica modified with HS-SiO2 thiol groups was utilized as adsorbent for solid phase extraction, as a fast and reliable method of preconcentration and separation of very small quantities of selenium ions from water and blood samples.
Methods: The samples included four natural water samples and one biological sample (blood serum) prepared in volumes of 25, 100, 200, 300, 400 and 500 ml. The samples were analyzed by solid phase microextraction, using thiolated-nanosilica (as adsorbent), ultraviolet-visible spectrophotometry and atomic absorption spectroscopy.
Results: Optimized conditions for preconcentration of a 25 ml 0.2 mg/l selenium solution were pH 5, 40 mg of adsorbent, sample-adsorbent mixing time of 15 minutes and 5 ml of 2N sulfuric acid as detergent. The volume limit and concentration factor were 400 and 80, respectively. Limit of detection and relative standard deviation of the method were 0.46 μg/l and 0.9%, respectively.
Conclusion: This study is the first to successfully utilize thiolated nanosilica for measuring low selenium levels. Thiolation of the absorbent increases selenium adsorption by thiolated-silica compared to SiO2.
Keywords: Solid phase extraction, Selenium, Preconcentration, Nano, UV-visible spectrophotometry.
Mahboubeh Tajaldini, Firooz Samadi, Ayyoob Khosravi, Azim Ghasemnejad, Jahanbakhsh Asadi,
Volume 14, Issue 2 (3-2020)
Abstract
ABSTRACT
Background and Objectives: Citrus fruits and their constituents especially naringin (NR), a natural predominant flavanone, have a wide range of pharmacological activities without toxicity against cancer cells. The aim of this study was to investigate the anticancer effects of orange peel extract (OPE) and naringin (NR) on esophageal squamous cell carcinoma (ESCC) cells.
Methods: Amount of phenol, flavonoid and antioxidants in OPE was determined using Folin-Ciocalteu procedure, aluminum chloride colorimetric and DPPH assays, respectively. Effects of NR and OPE on viability, wound healing assay and DNA fragmentation using DAPI were investigated. Data were analyzed by ImageJ software and GraphPad Prism 6.0 at significance of 0.05.
Results: Total amount of phenols, flavonoids and 1,1-diphenyl-2-picrylhydrazyl was 2.83, 2.143 and 60.76 g/100g of OPE. Amount of NR in the dried OPE was estimated to be 5.260 (µg/gr) using high-performance liquid chromatography. Treatment of ESCC cells with OPE or NR decreased viability y of cancer cells in a dose-dependent manner. In addition, both OPE and NR were able to decrease cell migration and increase DNA fragmentation.
Conclusion: The findings of our study suggest that OPE and NR have anticancer effects on ESCC cells but the anticancer effects of OPE was better than that of NR alone.
Keywords: Orange peel extract, Naringin, Migration, Esophageal squamous cell carcinoma.
Masoud Soosaraei, Ahmad Daryani, Shahabeddin Sarvi, Mohamad Taghi Rahimi, Mahdi Fakhar, Hajar Ziaei Hezarjaribi, Mehdi Sharif,
Volume 14, Issue 5 (9-2020)
Abstract
Background and objectives: Intestinal parasitic infections are a major public health problem worldwide, especially in developing countries. It is estimated that around 3.5 billion people are infected with intestinal parasites. Human intestinal parasites (HIP) are clinically important due to broad epidemiological distribution, reinfection and drug resistance. In the last decades, bioactive compounds from herbs were used against a wide variety of microorganisms including parasites. We aimed to perform a systematic review on studies on the effects of medicinal herbs on HIPs in Iran.
Methods: Relevant scientific publications until April, 2015 were extracted from five English databases (PubMed, Google Scholar, Ebsco, Science Direct and Scopus) and four Persian databases (Magiran, Irandoc, IranMedex and the Scientific Information Database).
Results: A total of 18 papers and two
dissertations met the inclusion criteria. Overall, 22 different plant extracts were used against
Giardia lamblia,
Entamoeba histolytica,
Cryptosporidium and
Hymenolepis nana. Based on the results, the extracts could exert time- and dose-dependent inhibitory effects against the tested HIPs. Five plants types including
Allium,
Chenopodium botrys, Carum copticum, F. asafoetida and
Artemisia annua were able to completely inhibit the tested parasites, while
Thymus vulgaris and
A. paradoxum showed the lowest inhibitory effect (7%).
Conclusions: Given the findings, it is recommended to conduct in vivo studies on medicinal herbs with favorable in vitro effects against HIPs.
Zynab Badeli, Phd Masoud Haghkhah, Ezzat Allah Ghaemi,
Volume 16, Issue 1 (1-2022)
Abstract
Background and objectives: Garlic is a medicinal plant with various health promoting properties including antimicrobial effects. In this study, we investigated in vitro antibacterial effects of garlic hydro-alcoholic extract against enterohemorrhagic Escherichia coli (EHEC).
Methods: Garlic hydro-alcoholic extract was prepared by maceration method. Phytochemical analysis of the extract was carried out using gas chromatography-mass spectrometry. Minimum inhibitory concentration (MIC) of the extract against EHEC was determined by micro-dilution assay. Cytotoxic effect of the garlic extract on human colon adenocarcinoma cell line (SW480) was assessed using MTT assay. Micro-dilution assay was also used to determine the MIC of the extract against EHEC when co-cultured with SW480 cells.
Results: The amount of organosulfur in garlic extract was 70.91% and the most common organosulfur compounds were trisulfide, di-2-propenyl (34.8%) and diallyl disulfide (14.83%). The MIC of garlic hydro-alcoholic extract on EHEC alone and when co-cultured with SW480 was 12.5 mg/ml. Concentrations of 12.5 mg/ml and 25 mg/ml of the extract significantly reduced the viability of SW480 cells compared to control and concentration of 6.25 mg/ml of garlic extract (p <0.0001).
Conclusion: The garlic hydro-alcoholic extract has inhibitory effects on EHEC in vitro. Therefore, it can be considered a suitable candidate for controlling infections caused by EHEC.
Marzieh Niakan, Habib Alla Johari, Mehrdad Shariati, Davood Moghadamnia, Ebrahim Talebi,
Volume 17, Issue 4 (7-2023)
Abstract
Marzieh Niakan1 
,
Habib Alla Johari 2,
Mehrdad Shariati1 ,
Davood Moghadamnia3 ,
Ebrahim Talebi4
Background: Research has shown that the extract of some plants has an effective protective effect on liver cells against toxins and oxidants. In this research, the effect of hydroalcoholic extract of ginger against hepatotoxicity caused by doxorubicin (Dox) in adult male rats was investigated.
Methods: In this experimental study, 63 adult male rats were divided into 7 groups (n = 9 per group). The experimental treatments included the control and placebo groups without the use of medicines, experimental group I that received Dox at a dose of 20 mg/kg, experimental groups II and III with 300 and 600 mg/kg of ginger hydroalcoholic extract, and experimental groups IV and V that received 20 mg/kg Dox + 300 and 600 mg/kg of ginger hydroalcoholic extract, respectively. After the end of the test period, serum levels of total bilirubin and direct bilirubin were measured. In addition, the histological changes in the liver were examined after hematoxylin and eosin (H&E) staining.
Results: Serum levels of total bilirubin and direct bilirubin in the Dox group showed a significant increase compared to the control group. In contrast, serum levels of total bilirubin and direct bilirubin in Dox + ZIN 600 and Dox + ZIN 300 had a significant reduction compared to the Dox group (P < 0.05). Ginger extract prevented apoptosis and Dox-induced liver tissue damage in dose-dependent designs.
Conclusion: The hydroalcoholic extract of ginger improves the changes of serum bilirubin and liver tissue after receiving Dox due to its antioxidant compounds.
