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Showing 2 results for Sotudeh
Chronic basophilic leukemia; A rare myeloproliferative disorder and the role of flow cytometry for its diagnosis
Saeid Anvari , Setare Kheyrandish , Fatemeh Sotudeh , Hossein Mirpour Hasankiadeh , Korosh Khanaki , Esmaeil Shahabi Satlsar,
Volume 19, Issue 2 (Mar-Apr 2025)
Abstract
Chronic basophilic leukemia (CBL) is a rare disorder and according to the published data, few cases have been reported as primary CBL. Morphologic findings in CBL mostly mimic chronic eosinophilic leukemia and basophils have unusual and dysplastic morphology. A 47-year-old patient was referred to the hospital with gastrointestinal symptoms and bloating. Complete blood count (CBC) showed leukocytosis and marked eosinophilia. After evaluating the peripheral blood smear (PBS), abnormal leukocytes with hypersegmented nuclei along with an increase in eosinophils were observed. For further investigation, the patient was referred to the hematology clinic and underwent bone marrow aspiration and biopsy. After the morphological examination, flow cytometry was performed on the aspiration sample to accurately diagnose the disease. Flow cytometric findings were in favor of CBL. Since morphological findings are unreliable for basophil detection and definite diagnosis, flow cytometry is a reliable method for the precise detection of basophils, especially in CBL.

Effects of ethanolic extract of Artemisia sieberi Besser on DNA glycation of glucose: Possible antidiabetic mechanism
Parisa Hasanein, Farnush Sotudeh, Mousa Bohlooli, Mohammad Haddadi,
Volume 19, Issue 5 (9-2025)
Abstract

Introduction: DNA Glycation damages DNA by inducing breaks of strands, mutations, and finally changes in gene expression, which is assumed as a main factor in pathogenesis of diabetes and its complications. Therefore, antiglycation agents have been focused recently for preventing and alleviating diabetes complications. According to the reported antidiabetic effects of Artemisia sieberi (A. sieberi) leaves extract, this study was aimed to determine the effect of ethanoic extract of A. sieberi on glucose-mediated DNA glycation for the first time.

Methods: DNA incubated with glucose in the presence or absence of A. sieberi for 4 weeks. The inhibitory or fascilitatory effects of A. sieberi on DNA structural changes were studied by various techniques. These techniques were included UV–Vis, fluorescence spectroscopy, circular dichroism (CD) and agarose gel electrophoresis.
Results: The findings of UV–Vis and fluorescence spectroscopy showed that A. sieberi decreased the DNA-AGE (advanced glycation end products) formation. Based on the CD and agarose gel electrophoresis results, the structural changes of glycated DNA was decreased in the presence of A. sieberi.
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